Semen Preparation for Artificial Insemination

Sperm Washing – Sperm Preparation for Intrauterine Insemination (IUI)

 

Sperm washing is required prior to intrauterine insemination because it removes chemicals, which may cause adverse reactions in the uterus. It also enhances the fertilizing capacity of the sperm and is thus recommended in cases of immunologic, male-factor or unexplained infertility.

IMPORTANT:
Sexual abstinence of at least 2 days but not more than 5 days is recommended prior to collecting a specimen. Specimens can be collected at our facility or at home, provided that it does not take more than 1 hour to bring the sample to our facility. Specimen should be collected in sterile wide-mouth containers via dry masturbation. Containers are available at our laboratory or at drug stores known as urine specimen cups. Once the sample has been collected, the container must be kept upright with the lid tightly secured. If you produce the specimen at home, you need to keep the specimen at body temperature. We recommend placing the container if an inner pocket during cold weather. Allergies to antibiotics should be reported prior to washing.

 

Sperm Washing Technique

Sperm washing involves removing the seminal plasma and any cryoprotectant, if used, from the specimen prior to intrauterine insemination.

Fertility Center of California offers 3 sperm washing techniques:

• Basic Sperm Wash
• Premium Wash
• Swim-up Technique

Sperm washing can be performed on a single specimen prior to initiating intrauterine insemination therapy. A trial wash helps determine the sperm washing technique that will yield the best results.

 

Basic Sperm Wash

This technique uses dilution and centrifugation. A sperm wash solution containing antibiotics and protein supplements is added to the ejaculate. After repeated centrifugation, the seminal fluid is eliminated from the sample and the sperm cells are concentrated for insemination. This procedure takes 20 to 40 minutes.

 

Premium Wash

This method uses density gradient centrifugation to isolate and purify the motile sperm in order to obtain a sperm sample with a motility of at least 90%, depending on the initial quality of the sample. Different concentrations of Isolate (extremely dense fluid) are layered in a test tube in an ascending order of density (heaviest layer at the bottom). When a semen sample is placed upon the upper-most Isolate layer and centrifuged, any debris, round cells, non-motile and poor quality sperm remain in the top layers. Only the motile sperm are able to get through to the bottom layer and are then concentrated for use in artificial insemination. This procedure takes 1 hour. The Premium technique is excellent for any type of sample (fresh or frozen) and can help assess male factor fertility.

 

Swim-Up Technique

This technique uses sperm self-migration to obtain a sperm sample with a motility of at least 90%, depending on the initial quality of the sample. A layer of fresh media is gently added to the semen sample in such a way that most of the motile sperm will swim out of the sample and upward into the added media. These harvested motile sperm are subsequently used for insemination. This procedure takes 2 hours. Oligozoospermic men (men with low sperm counts), men with poorly motile sperm as well as men with male-factor infertility are not suitable candidates for this technique. Their sperm will have difficulty swimming up out of the pellet into the nutrient medium.

 

Washed Sperm Used for Intrauterine Insemination (IUI)

A washed specimen can be safely stored for a couple of hours prior to insemination. Fertility Center of California offers the service to keep the washed specimen safe in a special medium for up to 6 hours if there is a delay of the artificial insemination.

 

Semen Preparation Services and Fees

Basic Wash	$150
Premium / Gradient Wash	$200
Mini Premium / Gradient Wash	$170
Retrograde Wash	$250
Gender Pre-Selection (includes sperm wash)	$570
Specimen Thawing	$25